{"id":11389,"date":"2022-05-22T10:21:38","date_gmt":"2022-05-22T10:21:38","guid":{"rendered":"https:\/\/www.rhesusnegative.net\/staynegative\/?p=11389"},"modified":"2022-01-18T10:25:01","modified_gmt":"2022-01-18T10:25:01","slug":"etruscan-artifacts","status":"publish","type":"post","link":"https:\/\/www.rhesusnegative.net\/staynegative\/etruscan-artifacts\/","title":{"rendered":"Etruscan Artifacts"},"content":{"rendered":"<div style=\"padding-bottom:20px; padding-top:10px;\" class=\"hupso-share-buttons\"><!-- Hupso Share Buttons - https:\/\/www.hupso.com\/share\/ --><a class=\"hupso_toolbar\" href=\"https:\/\/www.hupso.com\/share\/\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/static.hupso.com\/share\/buttons\/share-medium.png?w=640&#038;ssl=1\" style=\"border:0px; padding-top:5px; float:left;\" alt=\"Share Button\"\/><\/a><script type=\"text\/javascript\">var hupso_services_t=new Array(\"Twitter\",\"Facebook\",\"Pinterest\",\"Linkedin\");var hupso_background_t=\"#EAF4FF\";var hupso_border_t=\"#66CCFF\";var hupso_toolbar_size_t=\"medium\";var hupso_twitter_via = \"datebytype\";var hupso_image_folder_url = \"\";var hupso_twitter_via=\"datebytype\";var hupso_url_t=\"\";var hupso_title_t=\"Etruscan Artifacts\";<\/script><script type=\"text\/javascript\" src=\"https:\/\/static.hupso.com\/share\/js\/share_toolbar.js\"><\/script><!-- Hupso Share Buttons --><\/div>\n<p>Recently, Vernesi et al. (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF12\">2004<\/a>) attempted to determine the variation in the first hypervariable segment (HVS-I) of mtDNA extracted from a number of Etruscan teeth. However, the rather unusual variation reported calls authenticity of the ancient mtDNA into question.<\/p>\n\n\n\n<p>Multiple occurrences of the same tandem mutation in lineages from disjoint haplogroups distinctly signal artifacts (Bandelt et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF3\">2002<\/a>). For instance, the \u201cEtruscan\u201d data harbor the transition pair 16193-16219 thrice, on quite different HVS-I backgrounds, even separated by a restriction site\u2014once jointly with +14766&nbsp;<em>Mse<\/em>I and twice with \u221214766&nbsp;<em>Mse<\/em>I. The transition 16219, however, is quite a rare mutation, which is confined essentially to haplogroup U6ab (Maca-Meyer et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF7\">2003<\/a>) and to a specific subclade of haplogroup H that bears the two characteristic mutations 16482 and 239. In the latter case, one indeed finds the motif 16193-16219-16362 in the worldwide database\u2014for example, in Great Britain (Richards et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF10\">2000<\/a>), in Germany (Pfeiffer et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF9\">1999<\/a>), and in the United States (as recorded in the SWGDAM database [Monson et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF8\">2002<\/a>]); however, in the study by Vernesi et al. (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF12\">2004<\/a>), the motif 16193-16219 occurs without 16362.<\/p>\n\n\n\n<p>The mutation 16069, which almost always signals haplogroup J, is not seen in the \u201cEtruscan\u201d mtDNA data in combination with the 16126 transition or with +14766&nbsp;<em>Mse<\/em>I but is recorded twice with \u221214766&nbsp;<em>Mse<\/em>I. Could this surprising feature be explained by recurrent mutations at 16069? Hardly\u2014the 16069 transition is not among the \u201cspeedy\u201d transitions reported by Bandelt et al. (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF3\">2002<\/a>). The mtDNA lineages with motif 16126 (outside haplogroup H), 16126-16193, and 16126-16193-16278 are normally seen together with 16069 in the West Eurasian mtDNA pool (except in the evidently flawed HVS-I data of Fraumene et al. [<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF5\">2003<\/a>]). Therefore, we have to expect at least four independent mutations at site 16069 in the \u201cEtruscan\u201d mtDNA data\u2014if we do not want to invoke de novo mutations at 16193 and 16278 (and this without a single trace of any familiar haplogroup J lineage in this data set). The 16069 transition on non-J lineages has been observed earlier in the \u201cLadins\u201d (Stenico et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF11\">1996<\/a>), where it occurs three times (in tandem with the 16085 transition). The variation at 16069 in these data sets thus seems abnormal in comparison with the worldwide mtDNA database.<\/p>\n\n\n\n<p>Vernesi et al. (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF12\">2004<\/a>) rejected the idea that postmortem damage could have been responsible for the assumed back mutations at 16069 (or 16294), with reference to the study of Gilbert et al. (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF6\">2003<\/a>), inasmuch as no such case was observed there. This argument is, however, invalid, since the regular haplogroup J (or T, respectively) nucleotide T at 16069 (or 16294, respectively) is less frequent by 1 order of magnitude than the corresponding majority nucleotide C (of the Cambridge reference sequence), so that no significant inference could be drawn from Gilbert et al. (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF6\">2003<\/a>) as it was for asymmetric transition probabilities (C\u2192T vs. T\u2192C) at these sites.<\/p>\n\n\n\n<p>There are numerous technical flaws in figure 2 and table 1 of Vernesi et al. (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF12\">2004<\/a>)\u2014in addition to the potential sequencing problems\u2014thus additionally undermining confidence in their ancient mtDNA data. Haplotype 6AM is not well defined, because the corresponding HVS-I sequences (from Adria and Magliano\/Marsiliana [Vernesi et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF12\">2004<\/a>]) are observed with both +\/\u221214766&nbsp;<em>Mse<\/em>I, according to their table 1. Haplotype 13C is misplaced in figure 2, since it bears +14766&nbsp;<em>Mse<\/em>I. This figure certainly does not present a reduced median network (as claimed), because the Adria 6AM haplotype should be a neighbor of haplotype 14CMT. It is not at all clear how this network was actually constructed, because, in the article, the reference is given to the median-joining algorithm (Bandelt et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF1\">1999<\/a>), which is fundamentally different from the reduced-median algorithm (Bandelt et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF2\">2000<\/a>). The median-joining algorithm, however, would instead reconstruct a triangle for site 16095, in this case. In the data set, the 16223 transition relative to rCRS is observed twice in connection with \u221214766&nbsp;<em>Mse<\/em>I, which otherwise should be more the exception than the rule, since rCRS is the ancestral HVS-I motif of haplogroup HV (as well as of the superhaplogroup R). On the other hand, this restriction site has not been determined for haplotype 9A, yet it is reconstructed in the network, not most parsimoniously, as +14766&nbsp;<em>Mse<\/em>I. Finally, the node sizes in figure 2 do not always correspond to the frequencies recorded in table 1 of the article.<\/p>\n\n\n\n<p>The assertion that \u201call the strictest criteria for the validation of ancient DNA sequences have been followed\u201d (Vernesi et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF12\">2004<\/a>&nbsp;[p. 703]) is not quite correct, since one of the most important criteria of Cooper and Poinar (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF4\">2000<\/a>)\u2014that is, that of independent replication in another lab\u2014has not been followed for 25 of 28 of the reported HVS-I sequences or for any of the RFLP tests. Moreover, the 20 excluded sequences were not displayed. The claim that the \u201cEtruscan\u201d sequences \u201call belong to lineages that are still present in Europe\u201d (Vernesi et al.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1182123\/#RF12\">2004<\/a>&nbsp;[p. 702]) is not justified, in view of the unusual mutational pattern, especially as the basal haplogroup status (U, JT, pre-HV, N1, W, X, or other) was not determined in half of the data set. Under these circumstances, it is unclear to what extent the \u201cEtruscan\u201d data represent severely damaged or partly contaminated mtDNA sequences; therefore, any comparison with modern population data must be considered quite hazardous.<\/p>\n\n\n\n<figure class=\"wp-block-embed-wordpress wp-block-embed is-type-wp-embed is-provider-rh-negative-blood-and-people\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"vWbFTaX9pO\"><a href=\"https:\/\/www.rhesusnegative.net\/staynegative\/etruscans\/\">Were the ancient Etruscans an important link to rhesus negatives today?<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; clip: rect(1px, 1px, 1px, 1px);\" title=\"&#8220;Were the ancient Etruscans an important link to rhesus negatives today?&#8221; &#8212; The Rh Negative Blog\" src=\"https:\/\/www.rhesusnegative.net\/staynegative\/etruscans\/embed\/#?secret=NASdB3eCJS#?secret=vWbFTaX9pO\" data-secret=\"vWbFTaX9pO\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n<div style=\"padding-bottom:20px; padding-top:10px;\" class=\"hupso-share-buttons\"><!-- Hupso Share Buttons - https:\/\/www.hupso.com\/share\/ --><a class=\"hupso_toolbar\" href=\"https:\/\/www.hupso.com\/share\/\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/static.hupso.com\/share\/buttons\/share-medium.png?w=640&#038;ssl=1\" style=\"border:0px; padding-top:5px; float:left;\" alt=\"Share Button\"\/><\/a><script type=\"text\/javascript\">var hupso_services_t=new Array(\"Twitter\",\"Facebook\",\"Pinterest\",\"Linkedin\");var hupso_background_t=\"#EAF4FF\";var hupso_border_t=\"#66CCFF\";var hupso_toolbar_size_t=\"medium\";var hupso_twitter_via = \"datebytype\";var hupso_image_folder_url = \"\";var hupso_twitter_via=\"datebytype\";var hupso_url_t=\"\";var hupso_title_t=\"Etruscan Artifacts\";<\/script><script type=\"text\/javascript\" src=\"https:\/\/static.hupso.com\/share\/js\/share_toolbar.js\"><\/script><!-- Hupso Share Buttons --><\/div>","protected":false},"excerpt":{"rendered":"<div style=\"padding-bottom:20px; padding-top:10px;\" class=\"hupso-share-buttons\"><!-- Hupso Share Buttons - https:\/\/www.hupso.com\/share\/ --><a class=\"hupso_toolbar\" href=\"https:\/\/www.hupso.com\/share\/\"><img src=\"https:\/\/static.hupso.com\/share\/buttons\/share-medium.png\" style=\"border:0px; padding-top:5px; float:left;\" alt=\"Share Button\"\/><\/a><script type=\"text\/javascript\">var hupso_services_t=new Array(\"Twitter\",\"Facebook\",\"Pinterest\",\"Linkedin\");var hupso_background_t=\"#EAF4FF\";var hupso_border_t=\"#66CCFF\";var hupso_toolbar_size_t=\"medium\";var hupso_twitter_via = \"datebytype\";var hupso_image_folder_url = \"\";var hupso_twitter_via=\"datebytype\";var hupso_url_t=\"\";var hupso_title_t=\"Etruscan Artifacts\";<\/script><script type=\"text\/javascript\" src=\"https:\/\/static.hupso.com\/share\/js\/share_toolbar.js\"><\/script><!-- Hupso Share Buttons --><\/div>Recently, Vernesi et al. (2004) attempted to determine the variation in the first hypervariable segment (HVS-I) of mtDNA extracted from a number of Etruscan teeth. However, the rather unusual variation reported calls authenticity of the ancient mtDNA into question. Multiple occurrences of the same tandem mutation in lineages from disjoint haplogroups distinctly signal artifacts (Bandelt et al.&nbsp;2002). For instance, the \u201cEtruscan\u201d data harbor the transition pair 16193-16219 thrice, on quite different HVS-I backgrounds, even separated by a restriction site\u2014once jointly with +14766&nbsp;MseI and twice with \u221214766&nbsp;MseI. The transition 16219, however, is quite a rare mutation, which is confined essentially to haplogroup U6ab (Maca-Meyer et al.&nbsp;2003) and to a specific subclade of haplogroup H that bears the two characteristic mutations 16482 <div style=\"padding-bottom:20px; padding-top:10px;\" class=\"hupso-share-buttons\"><!-- Hupso Share Buttons - https:\/\/www.hupso.com\/share\/ --><a class=\"hupso_toolbar\" href=\"https:\/\/www.hupso.com\/share\/\"><img src=\"https:\/\/static.hupso.com\/share\/buttons\/share-medium.png\" style=\"border:0px; padding-top:5px; float:left;\" alt=\"Share Button\"\/><\/a><script type=\"text\/javascript\">var hupso_services_t=new Array(\"Twitter\",\"Facebook\",\"Pinterest\",\"Linkedin\");var hupso_background_t=\"#EAF4FF\";var hupso_border_t=\"#66CCFF\";var hupso_toolbar_size_t=\"medium\";var hupso_twitter_via = \"datebytype\";var hupso_image_folder_url = \"\";var hupso_twitter_via=\"datebytype\";var hupso_url_t=\"\";var hupso_title_t=\"Etruscan Artifacts\";<\/script><script type=\"text\/javascript\" src=\"https:\/\/static.hupso.com\/share\/js\/share_toolbar.js\"><\/script><!-- Hupso Share Buttons --><\/div>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"amp_status":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[1],"tags":[],"class_list":["post-11389","post","type-post","status-publish","format-standard","hentry","category-facts"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5DV68-2XH","_links":{"self":[{"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/posts\/11389","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/comments?post=11389"}],"version-history":[{"count":3,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/posts\/11389\/revisions"}],"predecessor-version":[{"id":11392,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/posts\/11389\/revisions\/11392"}],"wp:attachment":[{"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/media?parent=11389"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/categories?post=11389"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/tags?post=11389"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}