{"id":9450,"date":"2021-01-19T01:13:43","date_gmt":"2021-01-19T01:13:43","guid":{"rendered":"https:\/\/www.rhesusnegative.net\/staynegative\/?p=9450"},"modified":"2021-04-08T03:02:48","modified_gmt":"2021-04-08T03:02:48","slug":"rhd-gene-deletion-occurred-in-the-rhesus-box","status":"publish","type":"post","link":"https:\/\/www.rhesusnegative.net\/staynegative\/rhd-gene-deletion-occurred-in-the-rhesus-box\/","title":{"rendered":"RHD gene deletion occurred in the Rhesus box"},"content":{"rendered":"<div style=\"padding-bottom:20px; padding-top:10px;\" class=\"hupso-share-buttons\"><!-- Hupso Share Buttons - https:\/\/www.hupso.com\/share\/ --><a class=\"hupso_toolbar\" href=\"https:\/\/www.hupso.com\/share\/\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/static.hupso.com\/share\/buttons\/share-medium.png?w=640&#038;ssl=1\" style=\"border:0px; padding-top:5px; float:left;\" alt=\"Share Button\"\/><\/a><script type=\"text\/javascript\">var hupso_services_t=new Array(\"Twitter\",\"Facebook\",\"Pinterest\",\"Linkedin\");var hupso_background_t=\"#EAF4FF\";var hupso_border_t=\"#66CCFF\";var hupso_toolbar_size_t=\"medium\";var hupso_twitter_via = \"datebytype\";var hupso_image_folder_url = \"\";var hupso_twitter_via=\"datebytype\";var hupso_url_t=\"\";var hupso_title_t=\"RHD gene deletion occurred in the Rhesus box\";<\/script><script type=\"text\/javascript\" src=\"https:\/\/static.hupso.com\/share\/js\/share_toolbar.js\"><\/script><!-- Hupso Share Buttons --><\/div>\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" width=\"640\" height=\"998\" src=\"https:\/\/i0.wp.com\/www.rhesusnegative.net\/staynegative\/wp-content\/uploads\/2020\/02\/gene-deletion.png?resize=640%2C998&#038;ssl=1\" alt=\"\" class=\"wp-image-6238\" srcset=\"https:\/\/i0.wp.com\/www.rhesusnegative.net\/staynegative\/wp-content\/uploads\/2020\/02\/gene-deletion.png?resize=657%2C1024&amp;ssl=1 657w, https:\/\/i0.wp.com\/www.rhesusnegative.net\/staynegative\/wp-content\/uploads\/2020\/02\/gene-deletion.png?resize=192%2C300&amp;ssl=1 192w, https:\/\/i0.wp.com\/www.rhesusnegative.net\/staynegative\/wp-content\/uploads\/2020\/02\/gene-deletion.png?resize=768%2C1197&amp;ssl=1 768w, https:\/\/i0.wp.com\/www.rhesusnegative.net\/staynegative\/wp-content\/uploads\/2020\/02\/gene-deletion.png?w=2000&amp;ssl=1 2000w, https:\/\/i0.wp.com\/www.rhesusnegative.net\/staynegative\/wp-content\/uploads\/2020\/02\/gene-deletion.png?w=1280&amp;ssl=1 1280w, https:\/\/i0.wp.com\/www.rhesusnegative.net\/staynegative\/wp-content\/uploads\/2020\/02\/gene-deletion.png?w=1920&amp;ssl=1 1920w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><figcaption> <\/figcaption><\/figure><\/div>\n\n\n\n<p>The Rh blood group antigens derive from 2 genes, RHD and RHCE, that are located at chromosomal position 1p34.1-1p36 (chromosome 1, short arm, region 3, band 4, subband 1, through band 6). In whites, a cde haplotype with a deletion of the whole RHD gene occurs with a frequency of approximately 40%. The relative position of the 2 RH genes and the location of the RHD deletion was previously unknown. A model has been developed for the RH locus using RHD- and RHCE-related nucleotide sequences deposited in nucleotide sequence databases along with polymerase chain reaction (PCR) and nucleotide sequencing. The open reading frames of both RH genes had opposite orientations. The 3&#8242; ends of the genes faced each other and were separated by about 30 000 base pair (bp) that contained the SMP1 gene. The RHD gene was flanked by 2 DNA segments, dubbed Rhesus boxes, with a length of approximately 9000 bp, 98.6% homology, and identical orientation. The Rhesus box contained the RHD deletion occurring within a stretch of 1463 bp of identity. PCR with sequence-specific priming (PCR-SSP) and PCR with restriction fragment length polymorphism (PCR-RFLP) were used for specific detection of the RHD deletion. The molecular structure of the RH gene locus explains the mechanisms for generating RHD\/RHCE hybrid alleles and the RHD deletion. Specific detection of the RHD(-) genotype is now possible. (Blood. 2000;95:3662-3668)<\/p>\n\n\n\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/10845894\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/10845894\/<\/a><\/p>\n\n\n\n<p>See also:<\/p>\n\n\n\n<p><\/p>\n\n\n\n<figure class=\"wp-block-embed-wordpress wp-block-embed is-type-wp-embed is-provider-rh-negative-blood-and-people\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"pZzeHCS2u5\"><a href=\"https:\/\/www.rhesusnegative.net\/staynegative\/the-origin-of-rh-negative-blood\/\">The origin of Rh negative blood<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; clip: rect(1px, 1px, 1px, 1px);\" title=\"&#8220;The origin of Rh negative blood&#8221; &#8212; The Rh Negative Blog\" src=\"https:\/\/www.rhesusnegative.net\/staynegative\/the-origin-of-rh-negative-blood\/embed\/#?secret=E2OXss3Ycv#?secret=pZzeHCS2u5\" data-secret=\"pZzeHCS2u5\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<p> <\/p>\n<div style=\"padding-bottom:20px; padding-top:10px;\" class=\"hupso-share-buttons\"><!-- Hupso Share Buttons - https:\/\/www.hupso.com\/share\/ --><a class=\"hupso_toolbar\" href=\"https:\/\/www.hupso.com\/share\/\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/static.hupso.com\/share\/buttons\/share-medium.png?w=640&#038;ssl=1\" style=\"border:0px; padding-top:5px; float:left;\" alt=\"Share Button\"\/><\/a><script type=\"text\/javascript\">var hupso_services_t=new Array(\"Twitter\",\"Facebook\",\"Pinterest\",\"Linkedin\");var hupso_background_t=\"#EAF4FF\";var hupso_border_t=\"#66CCFF\";var hupso_toolbar_size_t=\"medium\";var hupso_twitter_via = \"datebytype\";var hupso_image_folder_url = \"\";var hupso_twitter_via=\"datebytype\";var hupso_url_t=\"\";var hupso_title_t=\"RHD gene deletion occurred in the Rhesus box\";<\/script><script type=\"text\/javascript\" src=\"https:\/\/static.hupso.com\/share\/js\/share_toolbar.js\"><\/script><!-- Hupso Share Buttons --><\/div>","protected":false},"excerpt":{"rendered":"<div style=\"padding-bottom:20px; padding-top:10px;\" class=\"hupso-share-buttons\"><!-- Hupso Share Buttons - https:\/\/www.hupso.com\/share\/ --><a class=\"hupso_toolbar\" href=\"https:\/\/www.hupso.com\/share\/\"><img src=\"https:\/\/static.hupso.com\/share\/buttons\/share-medium.png\" style=\"border:0px; padding-top:5px; float:left;\" alt=\"Share Button\"\/><\/a><script type=\"text\/javascript\">var hupso_services_t=new Array(\"Twitter\",\"Facebook\",\"Pinterest\",\"Linkedin\");var hupso_background_t=\"#EAF4FF\";var hupso_border_t=\"#66CCFF\";var hupso_toolbar_size_t=\"medium\";var hupso_twitter_via = \"datebytype\";var hupso_image_folder_url = \"\";var hupso_twitter_via=\"datebytype\";var hupso_url_t=\"\";var hupso_title_t=\"RHD gene deletion occurred in the Rhesus box\";<\/script><script type=\"text\/javascript\" src=\"https:\/\/static.hupso.com\/share\/js\/share_toolbar.js\"><\/script><!-- Hupso Share Buttons --><\/div>The Rh blood group antigens derive from 2 genes, RHD and RHCE, that are located at chromosomal position 1p34.1-1p36 (chromosome 1, short arm, region 3, band 4, subband 1, through band 6). In whites, a cde haplotype with a deletion of the whole RHD gene occurs with a frequency of approximately 40%. The relative position of the 2 RH genes and the location of the RHD deletion was previously unknown. A model has been developed for the RH locus using RHD- and RHCE-related nucleotide sequences deposited in nucleotide sequence databases along with polymerase chain reaction (PCR) and nucleotide sequencing. The open reading frames of both RH genes had opposite orientations. The 3&#8242; ends of the genes faced each other and <div style=\"padding-bottom:20px; padding-top:10px;\" class=\"hupso-share-buttons\"><!-- Hupso Share Buttons - https:\/\/www.hupso.com\/share\/ --><a class=\"hupso_toolbar\" href=\"https:\/\/www.hupso.com\/share\/\"><img src=\"https:\/\/static.hupso.com\/share\/buttons\/share-medium.png\" style=\"border:0px; padding-top:5px; float:left;\" alt=\"Share Button\"\/><\/a><script type=\"text\/javascript\">var hupso_services_t=new Array(\"Twitter\",\"Facebook\",\"Pinterest\",\"Linkedin\");var hupso_background_t=\"#EAF4FF\";var hupso_border_t=\"#66CCFF\";var hupso_toolbar_size_t=\"medium\";var hupso_twitter_via = \"datebytype\";var hupso_image_folder_url = \"\";var hupso_twitter_via=\"datebytype\";var hupso_url_t=\"\";var hupso_title_t=\"RHD gene deletion occurred in the Rhesus box\";<\/script><script type=\"text\/javascript\" src=\"https:\/\/static.hupso.com\/share\/js\/share_toolbar.js\"><\/script><!-- Hupso Share Buttons --><\/div>","protected":false},"author":1,"featured_media":6238,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"amp_status":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[1],"tags":[],"class_list":["post-9450","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-facts"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.rhesusnegative.net\/staynegative\/wp-content\/uploads\/2020\/02\/gene-deletion.png?fit=2000%2C3118&ssl=1","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5DV68-2sq","_links":{"self":[{"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/posts\/9450","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/comments?post=9450"}],"version-history":[{"count":3,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/posts\/9450\/revisions"}],"predecessor-version":[{"id":9995,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/posts\/9450\/revisions\/9995"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/media\/6238"}],"wp:attachment":[{"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/media?parent=9450"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/categories?post=9450"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.rhesusnegative.net\/staynegative\/wp-json\/wp\/v2\/tags?post=9450"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}