Can Rh negative blood be cloned?… another explanation:

Here is a random answer from Quora:

Red blood cells don’t have neuclei. There’s more room to carry haemoglobin and therefore oxygen without a neucleus, so red blood cells don’t have neuclei.

The neucleus of a cell contains the genetic material that is used in the cloning process. Since red blood cells don’t have any genetic material, they cannot be cloned. This is true of Rh positive cells and Rh negative cells. It’s the case for ALL red blood cells. Rh negative red blood cells are not special in this way – no red blood cell can be cloned.

Reticulocytes are immature red blood cells. Reticulocytes still have neuclei. Reticulocytes CAN be cloned, both Rh positive reticulocytes and Rh negative reticulocytes. In fact, it was by cloning reticulocytes and matching the gene sequences to the individuals’ phenotypes that the gene for the Rh D was identified. Researchers know which gene represents the D protein. Reticulocytes can be cloned whether or not they have this gene.

Molecular cloning of RhD cDNA derived from a gene present in RhD- positive, but not RhD-negative individualsAbstract. The Rh blood group system plays a major role in immune and nonimmune hemolytic states. Although an Rh cDNA has been previously cloned, there is no infhttps://ashpublications.org/blood/article/82/2/651/170408/Molecular-cloning-of-RhD-cDNA-derived-from-a-gene

On the other hand, the gene for Rh negative cannot be cloned. This is because it doesn’t exist. It’s a deletion, which means it isn’t there.

The abstract from above link:

The Rh blood group system plays a major role in immune and nonimmune hemolytic states. Although an Rh cDNA has been previously cloned, there is no information on which Rh antigenic protein it encodes. Using polymerase chain reaction (PCR) amplification, we have identified this original Rh clone, here designated Rh21, and an additional Rh cDNA clone, Rh13, that is 96% nucleotide- and 92% amino acid-identical to Rh21, with the substitutions scattered throughout the sequence. A molecular genetic approach was used to match this Rh clone with an Rh specificity. The mRNA transcript for Rh13 was present in reticulocytes from RhD-positive individuals, but was absent from the reticulocytes of RhD-negative individuals. Using conventional screening of genomic libraries, as well as PCR cloning, partial genomic clones for these two Rh cDNAs were obtained. Based on PCR analysis and Southern blots, the Rh21 gene was present in all individuals, but an intact Rh13 gene was only present in RhD-positive and not RhD-negative individuals. Thus, by correlating the presence of Rh mRNA and gene sequences with individual Rh phenotypes, we were able to establish that the new Rh13 cDNA clone represents the RhD protein.

My answer to the question “Can Rh negative blood be cloned?”