Home
Conversion of D-positive cells into D-negative is yet to be achieved

Conversion of D-positive cells into D-negative is yet to be achieved

 Rhesusnegative.net0
Share Button
Group O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a 12-transmembrane domain protein. Here we convert Rh D-positive erythroid progenitor cells into D-negative cells using RHD-targeting transcription activator-like effector nucleases (TALENs). After transfection of TALEN-encoding plasmids, RHD-knockout clones are obtained. Erythroid-lineage cells differentiated from these knockout erythroid progenitor cells do not agglutinate in the presence of anti-D reagents and do not express D antigen, as assessed using flow cytometry. Our programmable nuclease-induced blood group conversion opens new avenues for compatible donor cell generation in transfusion medicine.
Share Button

About The Author

Rhesusnegative.net

Disclaimer: This blog, all of its content and all of my posts are for Entertainment Purposes Only. Approval of comments does not equal agreement with content. Read at your own risk. Please note that comments will not be approved unless you use your real name. You can use first name only, if no one else has commented under that name before. Otherwise use first name and last initial. If that has been taken, use your full first and last name. No exceptions. Thank you for understanding.

Add a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.